Journal: The Journal of Biological Chemistry

Article Title: Small molecule intervention of actin-binding protein profilin1 reduces tumor angiogenesis in renal cell carcinoma

doi: 10.1016/j.jbc.2025.111033

Figure Lengend Snippet: Demonstration of C74’s ability to diminish tumor angiogenesis . A , chemical structure of C74. B - C , representative images ( panel B ; 4 × magnification) and quantification ( panel C ; based on 10 × field images) of CD31 + cells in subcutaneously established RENCA tumors subjected to daily direct intratumoral injection for 19 days with either C74 or equivalent amount of DMSO control in BALB/c mice (two-tailed Student’s t test, ∗∗, p < 0.01; n = 5 mice/group; the scale bar represents 200 μm). DMSO, dimethyl sulfoxide.

Article Snippet: RENCA renal cell carcinoma (RCC) cells (American Type Culture Collection [ATCC], CRL-2947) were cultured in RPMI supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin.

Techniques: Injection, Control, Two Tailed Test

Journal: The Journal of Biological Chemistry

Article Title: Small molecule intervention of actin-binding protein profilin1 reduces tumor angiogenesis in renal cell carcinoma

doi: 10.1016/j.jbc.2025.111033

Figure Lengend Snippet: Feasibility of ultrasound-guided tumor-localized delivery of Pfn1 inhibitor to achieve antiangiogenic and therapeutic benefit. A , experimental schema of tumor microbubble studies (MB–microbubbles). B , ultrasound images ( panel B ) showing MB infiltration into tumor vasculature ( white arrows ), followed by UTMD and no contrast remaining in the tumor space. C – J , results of C74-MB ( panels C through F ) and UP6-MB ( panels G – J ) tumor model studies. Images of subcutaneous RENCA tumors subjected to UTMD of indicated MBs ( panels C and G ) with end point tumor burden comparison between the control and experimental treatment groups ( panels D and H ). Representative H&E images of tumor histosections ( panels E and I; white arrows indicate functional tumor-associated blood vessels with presence of red blood cells) and the associated quantification ( panels F and I ) of the average number of tumor-associated blood vessels per 20X field of observation between the two treatment groups (Mann-Whitney test, ∗: p < 0.05; ∗∗ p < 0.01, ∗∗∗: p < 0.001; data pooled from 8-9 mice per group; the scale bar represents 50 μm). Pfn1, profilin1; UTMD, ultrasound-targeted microbubble destruction.

Article Snippet: RENCA renal cell carcinoma (RCC) cells (American Type Culture Collection [ATCC], CRL-2947) were cultured in RPMI supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin.

Techniques: Comparison, Control, Functional Assay, MANN-WHITNEY